Development of a quantitative method for biofilm analysis using image processing.

Development of a quantitative method for biofilm analysis using image processing.

What was the challenge or problem to solve?

The customer requested the development of a quantitative and automated method to evaluate biofilm growth on different surfaces.

Until now, the customer was using a qualitative method that was subjective and could be misleading when analyzing and classifying samples.

A quantitative method seeks to avoid these problems, since the results will become objective. In addition, if it is automated, it will allow a large number of samples to be analyzed efficiently in a short time.

Imagen2 Development of a quantitative method for biofilm analysis using image processing.
Imagen3 Development of a quantitative method for biofilm analysis using image processing.

How was it addressed or what was the solution?

To address the problem, the Forensic Engineering team decided to use ImageJ image processing software to analyze biofilm photos obtained by epifluorescence microscopy.

Using this program, a specific macro was developed to obtain a percentage of area covered by biofilm, thus providing quantitative results.

In this way, the customer will have a new method of analysis that will allow him to obtain objective results of his samples, in a reduced time, and with the possibility of avoiding artifacts that falsify the results.

Challenge

  • Develop a biofilm analysis method that provides quantitative results.

Solution

  • Use of ImageJ image processing software.
  • Design of a macro to obtain the percentage of area covered by biofilm.

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